Protein_Domain

Part:BBa_K1733002:Design

Designed by: Jeffrey Chen   Group: iGEM15_BostonU   (2015-09-17)


PYL dimerization domain


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 282
    Illegal XhoI site found at 269
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 325
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

PYL was originally biobrick compatible. We used a PCR reaction and ligated it into the psb1c3 backbone.

Source

Arabidopsis thaliana

References